Human Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) ELISA Kit

Mfr.No.

DL-MCL1-Hu

Antigen

Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1)

Alternative Names

EAT ; MCL1L ; MCL1S ; BCL2L3 ; TM ; Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 ; Bcl-2-like protein 3 ; Bcl-2-related protein EAT/mcl1 ; mcl1/EAT

Categories

Elisa Kit

Description

We are producing ELISA Kit for research use only. This immunoassay kit allows for the specific measurement of this index in serum, plasma,tissue homogenates, cell culture supernates or other biological fluids.

Specificity

This assay has high sensitivity and excellent specificity for detection of this index.
No significant cross-reactivity or interference between this index and analogues was observed.
Note:
Limited by current skills and knowledge, it is impossible to perform all possible cross-reactivity detection tests between this index and all analogues, therefore, cross reactivity may still exist.

Stability

The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.
Note:
To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

Conjugate

Biotin

Analytical Method

Quantitative

Assay Procedure Summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Caution

The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this reagent.

Certified

CE&ISO

Detection Method

Colorimetric

Detection Range

0.312-20ng/mL

Experimental Method

sandwich

Express System

E. coli

Introduction

Item	Standard		Test
Description	The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of MCL1 in human tissue homogenates, cell lysates or other biological fluids.		Conform
Identification	Colorimetric		Positive
Composition	Traditional ELISA Kit	Ready-to-Use ELISA KIT	Conform
	Pre-coated, ready to use 96-well strip plate 1	Pre-coated, ready to use 96-well strip plate 1
	Plate sealer for 96 wells 2	Plate sealer for 96 wells 2
	Standard 2	Standard 2
	Diluents buffer 1×45mL	Standard Diluent 1×20mL
	Detection Reagent A 1×120μL	Detection Solution A 1×12mL
	Detection Reagent B 1×120μL	Detection Solution B 1×12mL
	TMB Substrate 1×9mL	TMB Substrate 1×9mL
	Stop Solution 1×6mL	Stop Solution 1×6mL
	Wash Buffer (30 × concentrate) 1×20mL	Wash Buffer (30 × concentrate) 1×20mL
	Instruction manual 1	Instruction manual 1

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MCL1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1：4	1：8	1：16
serum(n=5)	82-96%	83-98%	81-99%	93-101%
EDTA plasma(n=5)	88-101%	86-95%	90-102%	80-93%
heparin plasma(n=5)	80-91%	82-90%	95-104%	79-95%

Overview

Other names:EAT ; MCL1L; MCL1S; BCL2L3; TM; Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; mcl1/EAT
Function:
nvolved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
Sequence:
MFGLKRNAVI GLNLYCGGAG LGAGSGGATR PGGRLLATEK
EASARREIGG GEAGAVIGGS AGASPPSTLT PDSRRVARPP
PIGAEVPDVT ATPARLLFFA PTRRAAPLEE MEAPAADAIM
SPEEELDGYE PEPLGKRPAV LPLLELVGES GNNTSTDGSL
PSTPPPAEEE EDELYRQSLE IISRYLREQA TGAKDTKPMG
RSGATSRKAL ETLRRVGDGV QRNHETAFQG MLRKLDIKNE
DDVKSLSRVM IHVFSDGVTN WGRIVTLISF GAFVAKHLKT
INQESCIEPL AESITDVLVR TKRDWLVKQR GWDGFVEFFH
VEDLEGGIRN VLLAFAGVAG VGAGLAYLIR

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-assay: CV<10%
Inter-assay: CV<12%

Recovery

Matrices listed below were spiked with certain level of recombinant MCL1 and the recovery rates were calculated by comparing the measured value to the expected amount of MCL1 in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	87-101	94
EDTA plasma(n=5)	90-101	95
heparin plasma(n=5)	91-102	96

Restriction

This product is for research use only.

Sample Availability

Yes

Sample Type

tissue homogenates, cell lysates or other biological fluids.

Sensitivity

The minimum detectable dose of MCL1 is typically less than 0.149ng/mL.
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Shelf Life

12 months

Specifity

This assay has high sensitivity and excellent specificity for detection of MCL1.
No significant cross-reactivity or interference between MCL1 and analogues was observed.
Note:
Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between MCL1 and all the analogues, therefore, cross reaction may still exist.

Summary

	Traditional ELISA Kit	Ready-to-Use ELISA KIT
Product name:	Human Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) ELISA Kit
Method:	Sandwich
Synonyms:	EAT; MCL1L; MCL1S; BCL2L3; TM; Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; mcl1/EAT
Detection range:	0.312-20ng/mL
Reactivity:	MCL1
Size:	96T/48T
Quality guarantee period:	for 12 months
Catalog number:	DL-MCL1-Hu(traditional)	DLR-MCL1-Hu(ready-to-use)
Assay length	1-4.5Hours	1-3.5Hours
Advantages:	Competitive price. High sensitivity. High stability. 12 months shelf life.	Pre-diluted Detection Reagent A and B Reduction in the number of steps when conducting the test All the reagents can be stored at 4℃ Faster reaction compare to other brands 12 months shelf life
Instruction Manual

Test Principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to MCL1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MCL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Gene Id	4170
Immunogen Species	Homo Sapiens (Human)
Uniprotkb	Q07820

Purity	Affinity Purification
Storage	4°C,-20°C

DL-MCL1-Hu
DLR-MCL1-Hu

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SKU	Supplier No.	Size	Your price
BHE13503800	DL-MCL1-Hu	Traditional--96T	$697.26	Add to Cart
BHE13503800	DL-MCL1-Hu	Traditional--48T	$503.26	Add to Cart
BHE13503800	DL-MCL1-Hu	Ready-to-Use--96T	$729.56	Add to Cart
BHE13503800	DL-MCL1-Hu	Ready-to-Use--48T	$525.86	Add to Cart

Human Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) ELISA Kit

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The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of MCL1 in human tissue homogenates, cell lysates or other biological fluids.

EAT; MCL1L; MCL1S; BCL2L3; TM; Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; mcl1/EAT

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