Human Interferon Regulatory Factor 2

Mfr.No.

E3333Hu

Sensitivity

9.47ng/L

Detection Range

20-3800ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Interferon regulatory factor 2;IRF 2;IRF2;IRF-2

Molecular Weight

39354

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3333Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	869.5	42.55	4.9
Intra-Assay	2	18	1197.9	50.97	4.3
Intra-Assay	3	18	332.4	15.8	4.8

Background

Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation.

Protocol

These standard curves of E3333Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	2.282	2.117	2.094
	1.951
1000ng/L	1.433	1.38	1.357
	1.327
500ng/L	0.761	0.659	0.636
	0.556
250ng/L	0.371	0.385	0.362
	0.399
125ng/L	0.197	0.195	0.172
	0.192
0ng/L	0.02	0.023	0
	0.025

Gene ID	3660
UniProt ID	P14316

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104862	E3333Hu	48T	$320		Add to Cart
BHE12104862	E3333Hu	96T	$458		Add to Cart

Human Interferon Regulatory Factor 2 - IRF2 ELISA Kit

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