Human Interferon Alpha/Beta Receptor 2 (IFNa/bR2) ELISA Kit

Mfr.No.

DL-IFNAR2-Hu

Alternative Names

IFNa/bR2 ; IFN-alpha-REC ; IFN-R ; IFNABR ; IFNARB ; Interferon(Alpha,Beta And Omega)Receptor Beta Chain ; Interferon alpha binding protein ; Type I interferon receptor 2

Categories

Elisa Kit

Description

We are producing ELISA Kit for research use only. This immunoassay kit allows for the specific measurement of this index in serum, plasma,tissue homogenates, cell culture supernates or other biological fluids.

Specificity

This assay has high sensitivity and excellent specificity for detection of this index.
No significant cross-reactivity or interference between this index and analogues was observed.
Note:
Limited by current skills and knowledge, it is impossible to perform all possible cross-reactivity detection tests between this index and all analogues, therefore, cross reactivity may still exist.

Stability

The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.
Note:
To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

Conjugate

Biotin

Analytical Method

Quantitative

Assay Procedure Summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Caution

The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this reagent.

Certified

CE&ISO

Detection Method

Colorimetric

Detection Range

0.625-40ng/mL

Experimental Method

sandwich

Express System

E. coli

Introduction

Item	Standard		Test
Description	The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of IFNa/bR2 in human serum, plasma, urine and other biological fluids.		Conform
Identification	Colorimetric		Positive
Composition	Pre-coated, ready to use 96-well strip plate	1	Conform
	Standard (freeze dried)	2
	Standard Diluent	1 × 20ml
	Detection Reagent A	1× 120μl
	Detection Reagent B	1× 120μl
	Assay Diluent A	1 × 12ml
	Assay Diluent B	1 × 12ml
	TMB Substhumane	1 × 9ml
	Stop Solution	1 ×6ml
	Wash Buffer(30 x concenthumane)	1 ×20ml
	Plate sealer for 96 wells	2
	Instruction manual	1

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFNa/bR2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1：4	1：8	1：16
serum(n=5)	82-96%	83-98%	81-99%	93-101%
EDTA plasma(n=5)	88-101%	86-95%	90-102%	80-93%
heparin plasma(n=5)	80-91%	82-90%	95-104%	79-95%

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-assay: CV<10%
Inter-assay: CV<12%

Recovery

Matrices listed below were spiked with certain level of recombinant IFNa/bR2 and the recovery rates were calculated by comparing the measured value to the expected amount of IFNa/bR2 in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	90-101	95
EDTA plasma(n=5)	91-100	95
heparin plasma(n=5)	90-99	94

Restriction

This product is for research use only.

Sample Availability

Yes

Shelf Life

12 months

Summary

Matrix	Recovery range (%)	Average(%)
serum(n=5)	90-101	95
EDTA plasma(n=5)	91-100	95
heparin plasma(n=5)	90-99	94

Test Principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to IFNa/bR2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to IFNa/bR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IFNa/bR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IFNa/bR2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Immunogen Species	Homo Sapiens (Human)

Purity	Affinity Purification
Storage	4°C,-20°C

DL-IFNAR2-Hu
DLR-IFNAR2-Hu

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SKU	Supplier No.	Size	Your price
BHE13502905	DL-IFNAR2-Hu	Traditional--96T	$613.86	Add to Cart
BHE13502905	DL-IFNAR2-Hu	Traditional--48T	$444.86	Add to Cart
BHE13502905	DL-IFNAR2-Hu	Ready-to-Use--96T	$642.06	Add to Cart
BHE13502905	DL-IFNAR2-Hu	Ready-to-Use--48T	$464.66	Add to Cart

Human Interferon Alpha/Beta Receptor 2 (IFNa/bR2) ELISA Kit

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The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of IFNa/bR2 in human serum, plasma, urine and other biological fluids.

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