Human Insulin-like Growth Factors Binding Protein 2

Mfr.No.

E0392Hu

Sensitivity

0.28ng/ml

Detection Range

0.5-100ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Insulin-like Growth Factors Binding Protein 2 (also known as IGFBP-2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

IBP-2;IGF-binding protein 2;IGFBP 2;IGFBP2;IGFBP-2;Insulin-like growth factor-binding protein 2

Molecular Weight

34814

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0392Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	34.2	0.93	2.7
Intra-Assay	2	18	22.9	1.16	5.1
Intra-Assay	3	18	12.5	0.87	6.9

Background

Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Protocol

These standard curves of E0392Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
64ng/ml	2.341	2.337	2.296
	2.332
32ng/ml	1.391	1.393	1.352
	1.395
16ng/ml	0.657	0.7	0.659
	0.743
8ng/ml	0.454	0.484	0.443
	0.514
4ng/ml	0.26	0.219	0.177
	0.177
0ng/ml	0.035	0.041	0
	0.048

Gene ID	3485
UniProt ID	P18065

Research Area	Signal Transduction; Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102088	E0392Hu	96T	$458		Add to Cart
BHE12102088	E0392Hu	48T	$320		Add to Cart

Human Insulin-like Growth Factors Binding Protein 2 - IGFBP2 ELISA Kit

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