Human Gtp Cyclohydrolase 1

Mfr.No.

E5366Hu

Alternative Names

GCH 1;GCH1;GTP cyclohydrolase 1;GTP cyclohydrolase I;GTP-CH-I

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

27903

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.47ng/L

Background

Positively regulates nitric oxide synthesis in umbilical vein endothelial cells (HUVECs). May be involved in dopamine synthesis. May modify pain sensitivity and persistence. Isoform GCH-1 is the functional enzyme, the potential function of the enzymatically inactive isoforms remains unknown.

Gene ID	2643
UniProt ID	P30793
Antigen	GCH1

Research Area

Others

Protocol

These standard curves of E5366Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	1.928	1.972	1.924
	2.016
800ng/L	0.898	0.907	0.858
	0.915
400ng/L	0.514	0.544	0.496
	0.574
200ng/L	0.166	0.222	0.173
	0.278
100ng/L	0.101	0.082	0.034
	0.064
0ng/L	0.04	0.049	0
	0.057

Precision

We measured random samples of E5366Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1295.7	37.97	2.9
Intra-Assay	2	18	985.6	40.17	4.1
Intra-Assay	3	18	401.8	20.32	5.1

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106729	E5366Hu	48T	$320		Add to Cart
BHE12106729	E5366Hu	96T	$458		Add to Cart

Human Gtp Cyclohydrolase 1 - GCH1 ELISA Kit

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