Human Dickkopf 3,DKK3 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E13607h

Alternative Names

dickkopf homolog 3 (Xenopus laevis), REIC, RIG, RIG-like 5-6, RIG-like 7-1, dickkopf 3, dickkopf homolog 3, regulated in glioma

Reactivity

Human

Detection Range

31.2 pg/mL-2000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Duration

1-5h

Recovery

The recovery of mouse SDF-1? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	95-101
EDTA plasma (n=4)	92	87-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SDF-1? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	101
	Range %	99-105
1:2	Average %	96
	Range %	91-98
1:4	Average %	89
	Range %	84-94
1:8	Average %	98
	Range %	95-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

7.8 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q9UBP4
Antigen	Dickkopf 3,DKK3

Applications

ELISA

Research Area

DKK3

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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