Matrices listed below were spiked with certain level of Complex I-6 and the recovery rates were calculated by comparing the measured value to the expected amount of Complex I-6 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-100
93
EDTA plasma(n=5)
87-105
95
heparin plasma(n=5)
92-105
100
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Complex I-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-105%
86-95%
92-102%
EDTA plasma(n=5)
85-96%
85-99%
87-98%
heparin plasma(n=5)
87-96%
86-94%
86-99%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
P03923
Applications
ELISA
Applications Notes
Complex I-6Elisa kit allows for the in vitro quantitative determination of Complex I-6 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human Complex I-6(NADH-ubiquinone oxidoreductase chain 6)Elisa kit