Human Cholesterol-25-Hydroxylase (CH25H) ELISA Kit

Mfr.No.

DL-CH25H-Hu

Alternative Names

h25OH ; Cholesterol 25-monooxygenase

Categories

Elisa Kit

Description

We are producing ELISA Kit for research use only. This immunoassay kit allows for the specific measurement of this index in serum, plasma,tissue homogenates, cell culture supernates or other biological fluids.

Specificity

This assay has high sensitivity and excellent specificity for detection of this index.
No significant cross-reactivity or interference between this index and analogues was observed.
Note:
Limited by current skills and knowledge, it is impossible to perform all possible cross-reactivity detection tests between this index and all analogues, therefore, cross reactivity may still exist.

Stability

The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.
Note:
To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

Conjugate

Biotin

Analytical Method

Quantitative

Assay Procedure Summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Caution

The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this reagent.

Certified

CE&ISO

Detection Method

Colorimetric

Detection Range

0.625-40ng/mL

Experimental Method

sandwich

Express System

E. coli

Introduction

Item	Standard		Test
Description	The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of CH25H in human serum, plasma, tissue homogenates and other biological fluids.		Conform
Identification	Colorimetric		Positive
Composition	Traditional ELISA Kit	Ready-to-Use ELISA KIT	Conform
	Pre-coated, ready to use 96-well strip plate 1	Pre-coated, ready to use 96-well strip plate 1
	Plate sealer for 96 wells 2	Plate sealer for 96 wells 2
	Standard 2	Standard 2
	Diluents buffer 1×45mL	Standard Diluent 1×20mL
	Detection Reagent A 1×120μL	Detection Solution A 1×12mL
	Detection Reagent B 1×120μL	Detection Solution B 1×12mL
	TMB Substrate 1×9mL	TMB Substrate 1×9mL
	Stop Solution 1×6mL	Stop Solution 1×6mL
	Wash Buffer (30 × concentrate) 1×20mL	Wash Buffer (30 × concentrate) 1×20mL
	Instruction manual 1	Instruction manual 1

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CH25H and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1：4	1：8	1：16
serum(n=5)	82-96%	83-98%	81-99%	93-101%
EDTA plasma(n=5)	88-101%	86-95%	90-102%	80-93%
heparin plasma(n=5)	80-91%	82-90%	95-104%	79-95%

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-assay: CV<10%
Inter-assay: CV<12%

Recovery

Matrices listed below were spiked with certain level of recombinant CH25H and the recovery rates were calculated by comparing the measured value to the expected amount of CH25H in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	83-100	91
EDTA plasma(n=5)	90-99	94
heparin plasma(n=5)	91-101	96

Restriction

This product is for research use only.

Sample Availability

Yes

Shelf Life

12 months

Summary

	Traditional ELISA Kit	Ready-to-Use ELISA KIT
Product name:	Human Cholesterol-25-Hydroxylase (CH25H) ELISA Kit
Method:	Sandwich
Synonyms:	h25OH; Cholesterol 25-monooxygenase
Detection range:	0.625-40ng/mL
Reactivity:	CH25H
Size:	96T/48T
Quality guarantee period:	for 12 months
Catalog number:	DL-CH25H-Hu(traditional)	DLR-CH25H-Hu(ready-to-use)
Assay length	1-4.5Hours	1-3.5Hours
Advantages:	Competitive price. High sensitivity. High stability. 12 months shelf life.	Pre-diluted Detection Reagent A and B Reduction in the number of steps when conducting the test All the reagents can be stored at 4℃ Faster reaction compare to other brands 12 months shelf life
Instruction Manual

Test Principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to CH25H. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CH25H. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CH25H, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CH25H in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Gene Id	9023
Immunogen Species	Homo Sapiens (Human)
Uniprotkb	O95992

Purity	Affinity Purification
Storage	4°C,-20°C

Manual

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SKU	Supplier No.	Size	Your price
BHE13501142	DL-CH25H-Hu	Traditional--96T	$697.26	Add to Cart
BHE13501142	DL-CH25H-Hu	Traditional--48T	$503.26	Add to Cart
BHE13501142	DL-CH25H-Hu	Ready-to-Use--96T	$729.56	Add to Cart
BHE13501142	DL-CH25H-Hu	Ready-to-Use--48T	$525.86	Add to Cart

Human Cholesterol-25-Hydroxylase (CH25H) ELISA Kit

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The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of CH25H in human serum, plasma, tissue homogenates and other biological fluids.

h25OH; Cholesterol 25-monooxygenase

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