Human Caspase-9,Casp-9 ELISA Kit

Human Caspase-9,Casp-9 ELISA Kit

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Mfr.No.

CSB-E08862h

Alternative Names

caspase 9, apoptosis-related cysteine peptidase, RP11-265F14.3, APAF-3, APAF3, CASPASE-9c, ICE-LAP6, MCH6, ICE-like apoptotic protease 6, apoptotic protease MCH-6, apoptotic protease activating factor 3, caspase 9, caspase 9, apoptosis-related cystein

Reactivity

Human

Detection Range

0.78 ng/mL-50 ng/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of pig sIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	103	98-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig sIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:200	Average %	92
	Range %	88-97
1:400	Average %	94
	Range %	90-98
1:800	Average %	87
	Range %	85-94
1:1600	Average %	96
	Range %	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

?g/ml	OD1	OD2	Average
37.5	0.120	0.115	0.118
9.38	0.456	0.463	0.460
2.34	0.901	0.877	0.889
0.58	1.432	1.527	1.480
0.15	2.103	2.189	2.146
0	2.544	2.753	2.649

Species

Homo sapiens

Sensitivity

0.195 ng/mL

Limit Of Detection

450 nm

UniProt ID	P55211
Antigen	caspase 9, apoptosis-related cysteine peptidase

Applications

ELISA

Research Area

CASP9

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501680	CSB-E08862h	96T	$595	Add to Cart
BHE10501680	CSB-E08862h	5x96T	$2856	Add to Cart
BHE10501680	CSB-E08862h	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.