Human Caspase 7 - CASP7 ELISA Kit

Mfr.No.

E2257Hu

Description

This sandwich kit is for the accurate quantitative detection of Human Cysteinyl Aspartate Specific Proteinases 7 (also known as CASPASE-7) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Apoptotic protease Mch-3;CASP 7;CASP7;CASP-7;Caspase-7;Caspase-7 subunit p11;Caspase-7 subunit p20;CMH-1;ICE-LAP3;ICE-like apoptotic protease 3

Reactivity

Human

Detection Range

30-9000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

34277

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

15.93ng/L

Background

Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Overexpression promotes programmed cell death.

Gene ID	840
UniProt ID	P55210
Antigen	CASP7

Research Area

Cell Biology

Protocol

These standard curves of E2257Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4800ng/L	2.296	2.248	2.183
	2.2
2400ng/L	1.48	1.45	1.385
	1.419
1200ng/L	0.882	0.826	0.761
	0.77
600ng/L	0.524	0.518	0.453
	0.512
300ng/L	0.287	0.287	0.222
	0.287
0ng/L	0.063	0.065	0
	0.067

Precision

We measured random samples of E2257Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2642.4	86.95	3.3
Intra-Assay	2	18	531.3	29.86	5.6
Intra-Assay	3	18	1062.4	66.41	6.3

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103797	E2257Hu	48T	$320		Add to Cart
BHE12103797	E2257Hu	96T	$458		Add to Cart

Human Caspase 7 - CASP7 ELISA Kit

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