Matrices listed below were spiked with certain level of beta-actin and the recovery rates were calculated by comparing the measured value to the expected amount of beta-actin in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-103
93
EDTA plasma(n=5)
85-97
91
heparin plasma(n=5)
85-103
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of beta-actin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
β-actin/ACTB/actin, beta/actin, cytoplasmic 1/beta cytoskeletal actin/Beta-actin/PS1TP5-binding protein 1/PS1TP5BP1
Notes
For Research Use Only
UniProt ID
P60709
Antigen
β-actin
Applications
ELISA
Applications Notes
beta-actin ELISA Kit allows for the in vitro quantitative determination of beta-actin concentrations in serum, plasma, tissue homogenates and other biological fluids.