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Home / ELISA Kit

Human Actin-like protein 6A (ACTL6A) ELISA Kit

SKU : BHE13703113

ACTL6A encodes a family member of actin-related proteins (ARPs), The 429-amino acid BAF53 protein shares extensive amino acid homology with actin and the yeast actin-related proteins Act2 and Act3. In addition, sequences encoding BAF53 homologs were identified in yeast and C. elegans. Two of 3 amino acids (asp11, gln137, and asp154 in actin) involved in tight calcium binding are conserved (asp17 and asp171 in BAF53).
In contrast with the overall homology to actin, the ATP-binding pocket in actin is poorly conserved in BAF53, suggesting that BAF53 may not have ATP-binding activity. A comparison of the sequences of actin family members revealed that BAF53 is most related to Arp3, which is involved in Listeria motility.

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Mfr.No.	KTE60897
Description	This Human Actin-like protein 6A (ACTL6A) ELISA Kit employs a two-site sandwich ELISA to quantitate ACTL6A.
Alternative Names	ACTL6A; ACTL6; ARPN-BETA; Arp4; BAF53A; INO80K; MGC5382; BAF complex 53 kDa subunit; BAF53; BRG1-associated factor; INO80 complex subunit K; actin-related protein 4; hArpN beta
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Actin-like protein 6A microplate Human Actin-like protein 6A standard Human Actin-like protein 6A detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Actin-like protein 6A (ACTL6A) ELISA Kit has high sensitivity and excellent specificity for detection of Human ACTL6A. No significant cross-reactivity or interference between Human ACTL6A and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	ACTL6A encodes a family member of actin-related proteins (ARPs), The 429-amino acid BAF53 protein shares extensive amino acid homology with actin and the yeast actin-related proteins Act2 and Act3. In addition, sequences encoding BAF53 homologs were identified in yeast and C. elegans. Two of 3 amino acids (asp11, gln137, and asp154 in actin) involved in tight calcium binding are conserved (asp17 and asp171 in BAF53). In contrast with the overall homology to actin, the ATP-binding pocket in actin is poorly conserved in BAF53, suggesting that BAF53 may not have ATP-binding activity. A comparison of the sequences of actin family members revealed that BAF53 is most related to Arp3, which is involved in Listeria motility.
Notes	This Human Actin-like protein 6A (ACTL6A) ELISA Kit employs a two-site sandwich ELISA to quantitate ACTL6A in samples. An antibody specific for ACTL6A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACTL6A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACTL6A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACTL6A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	86
Antigen	ACTL6A
Tag	ACTL6A
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Actin-like protein 6A (ACTL6A) ELISA Kit employs a two-site sandwich ELISA to quantitate ACTL6A in samples. An antibody specific for ACTL6A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACTL6A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACTL6A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACTL6A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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BHE13703113	KTE60897	48 T	$339		Add to Cart
BHE13703113	KTE60897	96 T	$589		Add to Cart
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BHE13703113	KTE60897	96 Tx50	$24569		Add to Cart

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