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Home / ELISA Kit

Human Actin, alpha skeletal muscle (ACTA1) ELISA Kit

SKU : BHE13703105

Actin alpha 1 which is expressed in skeletal muscle is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. Skeletal alpha actin expression is induced by stimuli and conditions known to cause muscle formation. Such conditions result in fusion of committed cells (satellite cells) into myotubes, to form muscle fibers. Skeletal actin itself, when expressed, causes expression of several other "myogenic genes", which are essential to muscle formation. One key transcription factor that activates skeletal actin gene expression is Serum Response Factor ("SRF"), a protein that binds to specific sites on the promoter DNA of the actin gene.

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Mfr.No.	KTE60889
Description	This Human Actin, alpha skeletal muscle (ACTA1) ELISA Kit employs a two-site sandwich ELISA to quantitate ACTA1.
Alternative Names	ACTA1; RP5-1068B5.2; ACTA; ASMA; CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3; alpha skeletal muscle actin
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Actin, alpha skeletal muscle microplate Human Actin, alpha skeletal muscle standard Human Actin, alpha skeletal muscle detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Actin, alpha skeletal muscle (ACTA1) ELISA Kit has high sensitivity and excellent specificity for detection of Human ACTA1. No significant cross-reactivity or interference between Human ACTA1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Actin alpha 1 which is expressed in skeletal muscle is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. Skeletal alpha actin expression is induced by stimuli and conditions known to cause muscle formation. Such conditions result in fusion of committed cells (satellite cells) into myotubes, to form muscle fibers. Skeletal actin itself, when expressed, causes expression of several other "myogenic genes", which are essential to muscle formation. One key transcription factor that activates skeletal actin gene expression is Serum Response Factor ("SRF"), a protein that binds to specific sites on the promoter DNA of the actin gene.
Notes	This Human Actin, alpha skeletal muscle (ACTA1) ELISA Kit employs a two-site sandwich ELISA to quantitate ACTA1 in samples. An antibody specific for ACTA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACTA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACTA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACTA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	58
Antigen	ACTA1
Tag	ACTA1
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Actin, alpha skeletal muscle (ACTA1) ELISA Kit employs a two-site sandwich ELISA to quantitate ACTA1 in samples. An antibody specific for ACTA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACTA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACTA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACTA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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