Matrices listed below were spiked with certain level of Acetylated p53 and the recovery rates were calculated by comparing the measured value to the expected amount of Acetylated p53 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-104
96
EDTA plasma(n=5)
92-105
97
heparin plasma(n=5)
94-105
100
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Acetylated p53 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Acetylated p53 ELISA Kit allows for the in vitro quantitative determination of Acetylated p53 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human Acetylated p53(Acetylated p53) ELISA Kit