Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Competitive
Recovery
Matrices listed below were spiked with certain level of T3 and the recovery rates were calculated by comparing the measured value to the expected amount of T3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-104
97
EDTA plasma(n=5)
94-105
99
heparin plasma(n=5)
89-100
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of T3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-105%
90-101%
86-104%
EDTA plasma(n=5)
84-97%
87-97%
85-101%
heparin plasma(n=5)
80-99%
86-91%
86-94%
Sensitivity
< 0.047ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
T3 ELISA Kit allows for the in vitro quantitative determination of T3 concentrations in serum, plasma, tissue homogenates and other biological fluids.