Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of GROalpha/CXCL1 and the recovery rates were calculated by comparing the measured value to the expected amount of GROalpha/CXCL1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-102
93
EDTA plasma(n=5)
87-104
96
heparin plasma(n=5)
90-103
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GROalpha/CXCL1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
92-97%
85-103%
90-105%
EDTA plasma(n=5)
88-100%
82-99%
82-99%
heparin plasma(n=5)
81-98%
84-99%
84-100%
Sensitivity
< 0.469pg/ml
Notes
For Research Use Only
UniProt ID
P14095
Applications
ELISA
Applications Notes
GROalpha/CXCL1 ELISA Kit allows for the in vitro quantitative determination of GROalpha/CXCL1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat GROalpha/CXCL1 (Growth Regulated Oncogene Alpha) ELISA Kit