Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of C3 and the recovery rates were calculated by comparing the measured value to the expected amount of C3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
84-105
97
EDTA plasma(n=5)
84-104
94
heparin plasma(n=5)
82-102
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-103%
81-101%
86-99%
EDTA plasma(n=5)
92-100%
91-100%
91-94%
heparin plasma(n=5)
91-100%
90-100%
90-95%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
UniProt ID
P01026
Applications
ELISA
Applications Notes
C3 ELISA Kit allows for the in vitro quantitative determination of C3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat C3 (Complement Component 3) ELISA Kit