Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of ADP/Acrp30 and the recovery rates were calculated by comparing the measured value to the expected amount of ADP/Acrp30 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-104
92
EDTA plasma(n=5)
88-102
93
heparin plasma(n=5)
85-105
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ADP/Acrp30 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-103%
89-101%
90-98%
EDTA plasma(n=5)
82-97%
86-94%
82-95%
heparin plasma(n=5)
80-100%
81-99%
80-99%
Sensitivity
< 1.875pg/ml
Notes
For Research Use Only
UniProt ID
Q6PP07
Applications
ELISA
Applications Notes
ADP/Acrp30 ELISA Kit allows for the in vitro quantitative determination of ADP/Acrp30 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Porcine ADP/Acrp30 (Adiponectin) ELISA Kit