Matrices listed below were spiked with certain level of 8-OHdG and the recovery rates were calculated by comparing the measured value to the expected amount of 8-OHdG in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-104
99
EDTA plasma(n=5)
95-102
100
heparin plasma(n=5)
88-105
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-OHdG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-101%
89-99%
90-104%
EDTA plasma(n=5)
90-97%
83-101%
84-98%
heparin plasma(n=5)
85-97%
81-100%
85-93%
Sensitivity
< 0.281ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
8-OHdG ELISA Kit allows for the in vitro quantitative determination of 8-OHdG concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse 8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit