Loading ....
High-Quality ELISA Kits for Accurate and Reliable Results
More than 100,000 ELISA kit, more than 15 selections of species
View All High Sensitivity ELISA Kits >>
High Sensitivity ELISA Kits
Signal Amplification Technology - High Sensitivity Accquant ELISA Kits
Our High Sensitivity ELISA Kits are engineered to detect and quantify low-abundance targets with unparalleled accuracy and reliability. These kits are ideal for measuring analytes present in minute concentrations across a variety of sample types, including serum, plasma, and cell culture supernatants. Compared to conventional ELISA kits, HS ELISA kits are tested and validated with more samples, resulting in improved sensitivity, reproducibility, and specificity.
Technology
Signal Amplification Technology: Utilizing biotinyl-tyramide signal amplification (TSA) technology, these kits enhance the signal generated by the enzyme horseradish peroxidase (HRP). This enables the detection of analytes at concentrations as low as 0.01 pg/mL, making the kits incredibly sensitive.
Optimized Antibodies: Featuring highly specific antibodies, our kits minimize background noise and increase assay precision.
Low Sample Volume: Only 50 μL of sample is required, making these kits ideal for studies with limited sample availability.
Ease of Use: Kits come with all necessary reagents, and the streamlined protocol ensures easy operation, minimizing errors and reducing hands-on time.
High Sensitivity ELISA Kits vs. Common ELISA Kits
To demonstrate the superiority of our Accquant ELISA kits, consider the example of human IL-6 detection:
| Feature | HS ELISA Kit (AQ-H0201-B) | Common ELISA Kit (EH0201) |
|---|---|---|
| Range | 0.078-5 pg/ml | 4.688-300 pg/ml |
| Sensitivity | 46 fg/ml | 2.813 pg/ml |
| Loading Amount | 50 µl | 100 µl |
| Sample Test Data (Healthy Serum) | 1.2-6 pg/ml | < 6 pg/ml, hard to detect |
High Sensitivity ELISA kits provide a broader detection range, higher sensitivity, and require a smaller sample volume compared to standard kits, allowing researchers to detect low-abundance proteins that would otherwise go unnoticed.
Side-by-side Comparison of the Different Types of Kits
| Feature | High Sensitivity ELISA Kits | Standard ELISA Kits | Competitive ELISA Kits | Radioimmunoassay (RIA) |
|---|---|---|---|---|
| Sensitivity | Very High As low as 0.01 pg/mL |
Moderate ng/mL range |
Lower Less sensitive |
High More sensitive than standard ELISA |
| Sample Volume | Low 50 μL |
Higher 100-200 μL |
Moderate Similar to standard |
Variable Depends on assay |
| Signal Amplification | Advanced Biotinyl-tyramide |
None | None | None, uses radioactivity |
| Detection Method | Sandwich Format With amplification |
Sandwich Format Without amplification |
Competitive Binding | Radioactive Isotopes |
| Specificity | Very High Optimized antibodies |
Moderate | Variable Depends on antibody quality |
High |
| Safety | Safe Non-radioactive |
Safe Non-radioactive |
Safe | Requires special handling |
| Cost and Convenience | Cost-effective Standard lab equipment |
Moderate | Moderate | Expensive Requires specialized equipment |
| Applications | Ideal for low-abundance targets Cytokines, biomarkers |
General applications | General applications | Highly sensitive but less practical |
HS ELISA Kits – More Sensitive, More Accurate
HS ELISA Kits: Taking Sensitivity to the Next Level
The High Sensitivity ELISA Kits are built on the foundation of the optimized AQ series, with significant improvements in sensitivity and performance. For frequently used ELISA kits, such as those targeting cytokines, the Accquant-HS series takes detection capabilities to the next level.
Case Study: Human IL-6 Detection
One of the most extensively studied cytokines is Interleukin-6 (IL-6). It plays a crucial role in regulating immune responses, acute phase reactions, and hematopoiesis. IL-6 is also vital for the body's defense mechanisms during infections. However, under normal physiological conditions, IL-6 levels are typically very low, making it difficult to detect using standard ELISA kits.
To address this challenge, FineTest developed the HS-IL-6 (High Sensitivity Interleukin-6) ELISA Kit. This kit is specifically designed to detect extremely low concentrations of IL-6, offering superior sensitivity for low-abundance protein detection compared to general ELISA kits.
Comparison: FineTest AQ(HS)-IL-6 vs. Market-Leading IL-6 ELISA Kits
| Vendor | Sensitivity | Range |
|---|---|---|
| Abcam | 0.8 pg/ml | 1.56 pg/ml – 50 pg/ml |
| R&D Systems | 90 fg/ml | 0.2 pg/ml – 10 pg/ml |
| FineTest® | 46 fg/ml | 0.078 pg/ml – 5 pg/ml |
Hot Selling HS ELISA Kits
|
Species
|
Cat.No
|
Product Name
|
Sensitivity
|
Range
|
|
Rat
|
BHE10808622
|
0.281ng/ml
|
0.468-30ng/ml
|
|
|
Human
|
BHE10807834
|
1.875pg/ml
|
3.1-200pg/ml
|
|
|
Mouse
|
BHE10808341
|
0.938pg/ml
|
1.563-100pg/ml
|
|
|
Mouse
|
BHE10807833
|
3.75pg/ml
|
6.25-400pg/ml
|
|
|
Human
|
BHE10807836
|
1.41pg/ml
|
2.343-150pg/ml
|
|
|
Human
|
BHE10807621
|
0.28pg/ml
|
0.46-30pg/ml
|
|
|
COVID-19
|
BHE10807647
|
390fg/ml
|
0.78-50pg/ml
|
|
|
Human
|
BHE10807832
|
0.046pg/ml
|
0.078-5pg/ml
|
|
|
Human
|
BHE10807835
|
0.469pg/ml
|
0.781-50pg/ml
|
|
|
Human
|
BHE10807838
|
0.875pg/ml
|
0.31-20pg/ml
|
|
|
Human
|
BHE10807841
|
0.983pg/ml
|
1.563-100pg/ml
|
|
|
Universal
|
BHE10808621
|
0.281ng/ml
|
0.468-30ng/ml
|
FineTest® ELISA Kits: Advancing Research with High Performance
Biohippo Partner with FineTest, dedicated to developing and optimizing ELISA kits based on real customer requirements. With years of experience in the field, we continually improve our products' performance to meet the evolving needs of researchers worldwide. The new Accquant series ELISA kits are a testament to our commitment to quality, offering superior sensitivity, reproducibility, and specificity compared to standard ELISA kits.
Why Choose FineTest® ELISA Kits?
Self-Produced Raw Materials for Quality Control: We maintain full control over our ELISA kits' quality by producing our own proteins and antibodies. This ensures consistent quality and performance across all batches.
Unique Signal Amplification Technology:Our proprietary signal amplification technology allows for flexible adjustment of kit sensitivity, improving the detection rate of your samples. This innovation enhances the overall accuracy of your results.
Advanced Sample Banks for Better Detection: With self-established cell and blood tissue sample banks, we provide a wide variety of samples for accurate detection and comprehensive testing.
GMP-Standard Production Facilities:Our kits are manufactured in GMP-standard workshops, maintaining a clean production environment that enhances product reliability and consistency.
High-Precision Production and Strict Quality Control (QC): We adhere to stringent quality control measures, ensuring a coefficient of variation (CV) of less than 5% for a wide range of samples.
Unique Stabilization Technology for Easy Storage and Transportation: Our ELISA kits are stabilized to maintain activity for up to a month, even at room temperature, making them more convenient to store and transport.
Learn Feature ELISA Kits at Biohippo
QuickTest ELISA Kits
With a streamlined protocol that delivers results in just 120 minutes, QuickTest ELISA Kits save valuable time in your research.
Learn MoreDiscover Our Extensive Range of QuickTest ELISA Kits
Our QuickTest ELISA Kits cover over 300 antigens, ensuring we have the right kit to meet your specific research needs.
Learn More
Cytokines ELISA Kits
Discover our extensive range of Cytokines ELISA kits, meticulously designed for the accurate and efficient detection of cytokines in various samples.
Learn More
ELISA Kit Promotions
Take advantage of our special promotions on ELISA kits, designed to provide you with high-quality products at discounted prices. Whether you’re conducting research or performing diagnostic tests, our promotional offers ensure you get the best value for your investment.
Learn More