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HIF1 alpha Antibody: RPE

BHA11901350
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Tel
+1 866.986.9598
Email
[email protected]
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Swissprot Q61221
Accession Number NP_034561.2
Gene Id 15251
Target HIF 1 alpha
Clone No ESEE122
Research Area Cancer, Oxidative Stress, Cell Signaling, Epigenetics and Nuclear Signaling
Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:1000), IHC (1:100), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response of hypoxia (1). The HIF1 complex consists of two subunits, HIF1-Alpha and HIF1-Beta, which are basic helix-loop-helix proteins of the PAS family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3, 4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).
Shipping Temperature Blue Ice or 4ºC
Specificity Detects ~116kDa. Specific for HIF1Alpha.
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Storage Temperature -20ºC
Applications ELISA, ICC, IHC, IF, WB
Description WB ; IHC ; ICC ; IF ; ELISA
Dilution WB (1:1000), IHC (1:100), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
Reference 1. Sharp F.R. and Bernaudin M. (2004) Nat Rev Neurosci 5: 437-48.
2. Wang G.L., et al. (1995) Proc Natl Acad Sci U S A 92: 5510-4.
3. Jaakkola P., et al. (2001) Science 292: 468-72.
4. Maxwell P.H., et al. (1999) Nature 399: 271-5.
5. Fukuda R., et al. (2002) J Biol Chem 277: 38205-11.
6. Jiang B.H., et al. (2001) Cell Growth Differ 12: 363-9.
7. Laughner E., et al. (2001) Mol Cell Biol 21: 3995-4004.
Conjugate Datasheet
SDS
SKU Supplier No. Size Your price Quantity
BHA11901350 SMC-184D-RPE 100µg $370.89
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