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Home / Proteins & Peptide

GM-CSF, RhesusMacaque

SKU : BHP18500055

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iXCells Biotechnologies

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Mfr.No.	IX02761, IX02761-1, IX02761-10
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <= -20 °C. Further dilutions should be made in appropriate buffered solutions.
Description	GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages and eosinophils.
Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Molecular Weight	Approximately 14.4 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Source	E. coli
Host	RhesusMacaque
Endotoxin Level	Less than 1 EU/ug of rRhGM-CSF as determined by LAL method.
Bioactivity	Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of 1.0 x 107 IU/mg.

Synonyms	Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), Rhesus Macaque;
Sequence	APARSPSPGT QPWEHVNAIQ EARRLLNLSR DTAAEMNKTVEVVSEMFDLQ EPSCLQTRLE?LYKQGLQGSL TKLKGPLTMM ASHYKQHCPPTPETSCATQI ITFQSFKENL KDFLLVIPFD?CWEPVQE

Purity	98 % by SDS-PAGE and HPLC analyses.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500055-1	IX02761-1	1mg	$2995		Add to Cart
BHP18500055-2	IX02761-10	10ug	$170		Add to Cart

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