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Home / Proteins & Peptide

GM-CSF, RhesusMacaque

SKU : BHP18500055

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iXCells Biotechnologies

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Mfr.No.	IX02761, IX02761-1, IX02761-10
Description	GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages and eosinophils.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <= -20 °C. Further dilutions should be made in appropriate buffered solutions.
Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Molecular Weight	Approximately 14.4 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Source	E. coli
Host	RhesusMacaque
Endotoxin Level	Less than 1 EU/ug of rRhGM-CSF as determined by LAL method.
Bioactivity	Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of 1.0 x 107 IU/mg.

Synonyms	Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), Rhesus Macaque;
Sequence	APARSPSPGT QPWEHVNAIQ EARRLLNLSR DTAAEMNKTVEVVSEMFDLQ EPSCLQTRLE?LYKQGLQGSL TKLKGPLTMM ASHYKQHCPPTPETSCATQI ITFQSFKENL KDFLLVIPFD?CWEPVQE

Purity	98 % by SDS-PAGE and HPLC analyses.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500055-1	IX02761-1	1mg	$2995		Add to Cart
BHP18500055-2	IX02761-10	10ug	$170		Add to Cart

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