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Home / Proteins & Peptide

GM-CSF, Human

SKU : BHP18500029

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iXCells Biotechnologies

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Mfr.No.	IX02695, IX02695-1, IX02695-10, IX02695-50
Description	Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF), a hematopoietic growth factor, is mainly involved in granulopoiesis and monocytopoiesis. It is produced by T-cells and macrophages in response to antigens, and by endothelial cells and fibroblasts following induction of variouscytokines (1). A monomeric protein of 127 amino acidswith six glycosylation sites and two intra disulfide bonds (2,3), glycosylated and non-glycosylated hGM-CSFs show similar biological activities (4).Other than its connection to the growth and development of granulocytes and macrophages, it is also indispensable for the proliferation of erythroid and megakaryocytic cells (5).Recombinant human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) produced in E. coli is a non-glycosylated polypeptide. A fully biologically active molecule, rhGM-CSF has a molecular mass of 14 kDa analyzed by SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques.
Reconstitution	Reconstituted in ddH2O at 100ug/ml.
Formulation	Lyophilized after extensive dialysis against 10 mM PB, pH7.0.
Molecular Weight	14 kDa, observed by SDS-PAGE.
Source	E. coli
Host	Human
Endotoxin Level	1 EU/ug, determined by LAL method.
Notes	For research use only
Bioactivity	ED50 0.5 ng/ml, measured by proliferation assay of TF-1 cells, corresponding to a specific activity of 2.0 x 106units/mg

Synonyms	Granulocyte Macrophage Colony Stimulating Factor, CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim, MGC131935, MGC138897
Sequence	MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNETVEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTMMASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPFDCWEPVQE

Purity	95 % as analyzed by SDS-PAGE.
Storage	Lyophilized recombinant human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) remains stable up to 12 months at lower than -70°C from date of receipt. Upon reconstitution, rhGM-CSF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500029-1	IX02695-1	1mg	$2621		Add to Cart
BHP18500029-2	IX02695-10	10ug	$107		Add to Cart
BHP18500029-3	IX02695-50	50ug	$344		Add to Cart

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