General species Guanosine (GS) (Competitive EIA) ELISA Kit

Mfr.No.

CEO134Ge

Description

This assay has high sensitivity and excellent specificity for detection of Guanosine (GS).
No significant cross-reactivity or interference between Guanosine (GS) and analogues was observed.

Alternative Names

Guanine Riboside

Reactivity

General

Detection Method

Colormetric

Detection Range

61.7-5,000pg/mL

Sample Type

Other biological fluids, Tissue homogenates, Cell lysates, Cell culture supernates, Serum, Plasma

Assay Type

Competitive Inhibition

Assay Duration

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
Reagent Diluent	1×300µL	Stop Solution	1×6mL
TMB Substrate	1×9mL	Instruction manual	1
Wash Buffer (30 × concentrate)	1×20mL

Features

Detects small molecule

Species

General species

Sensitivity

26.7pg/mL

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Notes

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Guanosine (GS) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Guanosine (GS) and unlabeled Guanosine (GS) (Standards or samples) with the pre-coated antibody specific to Guanosine (GS). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Guanosine (GS) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Guanosine (GS) in the sample.

Antigen	GS
Usage Notes	1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Applications	ELISA
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Guanosine (GS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Guanosine (GS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: C<10% Inter-Assay: C<12%

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

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SKU	Supplier No.	Size	Your price
BHE10108401	CEO134Ge	96T*1	$820	Add to Cart
BHE10108401	CEO134Ge	96T*5	$3690	Add to Cart
BHE10108401	CEO134Ge	96T*10	$6970	Add to Cart

ELISA Kit for Guanosine (GS) (General species)

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