General species Asymmetrical Dimethylarginine (ADMA) (Competitive EIA) ELISA Kit

Mfr.No.

CEB301Ge

Description

This assay has high sensitivity and excellent specificity for detection of Asymmetrical Dimethylarginine (ADMA).
No significant cross-reactivity or interference between Asymmetrical Dimethylarginine (ADMA) and analogues was observed.

Alternative Names

N,N-Dimethylarginine; NG,NG-Dimethylarginine dihydrochloride; Asymmetric Dimethylarginine

Reactivity

General

Detection Method

Colormetric

Detection Range

12.35-1,000ng/mL

Sample Type

Tissue homogenates, Cell lysates, Cell culture supernates, Other biological fluids, Serum, Plasma

Assay Type

Competitive Inhibition

Assay Duration

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120µL	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
TMB Substrate	1×9mL	Stop Solution	1×6mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

Recovery

Matrices listed below were spiked with certain level ofAsymmetrical Dimethylarginine (ADMA) and the recovery rates were calculated by comparing the measured value to the expected amount of Asymmetrical Dimethylarginine (ADMA) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	78-92	87
EDTA plasma(n=5)	92-101	96
heparin plasma(n=5)	96-103	101

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Asymmetrical Dimethylarginine (ADMA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	87-101%	85-97%	98-105%	93-101%
EDTA plasma(n=5)	86-96%	84-101%	91-98%	95-102%
heparin plasma(n=5)	85-102%	98-105%	83-92%	90-101%

Features

Detects small molecule

Species

General species

Sensitivity

4.99ng/mL

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Notes

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Asymmetrical Dimethylarginine (ADMA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Asymmetrical Dimethylarginine (ADMA) and unlabeled Asymmetrical Dimethylarginine (ADMA) (Standards or samples) with the pre-coated antibody specific to Asymmetrical Dimethylarginine (ADMA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Asymmetrical Dimethylarginine (ADMA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Asymmetrical Dimethylarginine (ADMA) in the sample.

Antigen	ADMA
Usage Notes	1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Applications	ELISA
Research Area	Metabolic pathway
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Asymmetrical Dimethylarginine (ADMA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Asymmetrical Dimethylarginine (ADMA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

Reference	Interactions among Vascular-Tone Modulators Contribute to High Altitude Pulmonary Edema and Augmented Vasoreactivity in Highlanders Effect of CCL5 on dimethylarginine dimethylaminohydrolase-1 production in vascular smooth muscle cells from spontaneously hypertensive rats. Correlation research on ADMA plasma levels and left ventricular function of peritoneal dialysis patients Effect of 18β-glycyrrhetinic acid on cerebral vasospasm caused by asymmetric dimethylarginine after experimental subarachnoid hemorrhage in rats. Asymmetric dimethylarginine contributes to airway nitric oxide deficiency in patients with COPD Dimethylarginine dimethylaminohydrolase-1 mediates inhibitory effect of interleukin-10 on angiotensin II-induced hypertensive effects in vascular smooth muscle cells of spontaneously hypertensive rats Effect of 18β-glycyrrhetinic acid on cerebral vasospasm caused by asymmetric dimethylarginine after experimental subarachnoid hemorrhage in rats Dimethylarginine Dimethylaminohydrolase 2 (DDAH 2) Gene Polymorphism, Asymmetric Dimethylarginine (ADMA) Concentrations, and Risk of Coronary Artery Disease: A Case-Control Study Dimethylarginine Dimethylaminohydrolase 2 (DDAH 2) Gene Polymorphism, Asymmetric Dimethylarginine (ADMA) Concentrations, and Risk of Coronary Artery Disease: A CaseControl Study Increased serum concentrations of asymmetric dimethylarginine (ADMA) in patients with early-onset coronary artery disease. Asymmetric dimethylarginine (ADMA) elevation and arginase up-regulation contribute toendothelial dysfunction related to insulin resistance in rats and morbidly obese humans.

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SKU	Supplier No.	Size	Your price
BHE10103758	CEB301Ge	96T*1	$808	Add to Cart
BHE10103758	CEB301Ge	96T*5	$3636	Add to Cart
BHE10103758	CEB301Ge	96T*10	$6868	Add to Cart

ELISA Kit for Asymmetrical Dimethylarginine (ADMA) (General species)

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