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Home / VECTOR &VIRUS / Lentivirus

Fluorescence and Luciferase Dual Reporter Lentivirus

SKU : BHV19400181

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LipExoGen

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Mfr.No.	LFV-0001, LFV-0001-01, LFV-0001-02, LFV-0001-03, LFV-0001-04, LFV-0001-05, LFV-0001-06, LFV-0001-07, LFV-0001-08, LFV-0001-09, LFV-0001-10, LFV-0001-11, LFV-0001-12, LFV-0001-13, LFV-0001-14
Description	Fluorescence and luciferase dual imaging reporter lentivirus: Collection of high-quality reporter lentiviral particles which allow for detection of transduced cells by luciferase and fluorescence based methods. The vector constructs contain self-cleaving peptides for independent protein expression of the luminescent (firefly luciferase) and fluorescent (GFP or RFP) reporters from a single mRNA transcript. The lentiviral particles are ultra-purified and concentrated to high-titer by PEG precipitation and sucrose gradient centrifugation, and are ideal for transduction of difficult-to-transfect cells including primary and/or thawed cells. These versatile lentiviral reporters provide an incredibly powerful tool for studying tumor growth and metastasis in preclinical models of cancer. Key Advantages: Dual-Readout - Get the most data from your experiments by exploiting luciferase and fluorescent reporters in the same cells. Easily transduce primary tumor cells or tumor cell lines and use luciferase imaging to track tumor growth and metastasis in vivo. Then, use the GFP or RFP reporter to identify the tumor cells on flow cytometry from dissociated tumors. The fluorescent reporters are excellent for gating out tumor cells for cleaner analysis of tumor-infiltrated lymphocytes (TILs), tumor associated macrophages (TAMs), myeloid-derived suppressor cells (MDSCs), and other infiltrated immune subsets. Alternatively, they can be used to sort or exclude tumor cells by FACS prior to scRNA-seq, proteomics analysis, and other downstream applications. Fluorescence microscopy can also be used to monitor living cells. Optimized Vectors - LipExoGen lentiviral particles are made using a novel vector platform based on the third generation system. Expression of the luminescent and fluorescent reporters and drug selection markers is driven by CMV, PGK, or EF1a promoter, and the genes are multicistronically expressed as individual proteins mediated by self-cleaving peptides. Multiple vector layouts are available, and different combinations of promoter, reporter, and drug selection are summarized in the table below. Ultra-Purified & High-Titer - Dual-imaging lentiviral particles are ultra-purified and concentrated to high-titer by PEG purification and sucrose gradient centrifugation, and are suitable for both in vitro and in vivo applications. Easily Transduce Primary Cells - Dual-imaging lentiviral particles can efficiently transduce primary tumor cells as well as bone marrow stem cells, lymphocytes, monocytes, neurons, and other difficult-to-transfect cells. The particles are also effective for transduction of freeze-thawed cells. Easily Establish Stable Cell Lines - Stable cell lines are easily generated through puromycin or blasticidin selection. Alternatively, transduced cells can be FACS sorted based on expression of GFP or RFP. Multiple vector layouts and combinations of promoter/reporter/drug selection are available, summarized below. Products with vector layouts LTV-V1 and LTV-V2 are the most effective for establishing stable cell lines. For applications where transduction of primary cells followed by FACS sorting is the primary focus, products with vector layouts LTV-V3 and LTV-V4 are recommended
Promoter	EF1a, CMV, PGK
Selection	Blasticidin, Puromycin
Vector Type	LFV-V1a, LFV-V1b, LFV-V3, LFV-V4
Virus Type	Luciferase and Fluorescence Dual Imaging Reporter Lentivirus, In vivo imaging tools

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Combination SKU	Supplier No.	Amount(TU)	Selection	Component arrangement	Promoter	Vector layout	Your price	Quantity
BHV19400181-1	LFV-0001-01	2x10^6	Puromycin	Luc-T2A-GFP-P2A-Puro	CMV	LFV-V1a	$595		Add to Cart
BHV19400181-2	LFV-0001-02	2x10^6	Puromycin	Luc-T2A-RFP-P2A-Puro	CMV	LFV-V1a	$595		Add to Cart
BHV19400181-3	LFV-0001-03	2x10^6	Blasticidin	Luc-T2A-GFP-P2A-BSD	CMV	LFV-V1a	$595		Add to Cart
BHV19400181-4	LFV-0001-04	2x10^6	Blasticidin	Luc-T2A-RFP-P2A-BSD	CMV	LFV-V1a	$595		Add to Cart
BHV19400181-5	LFV-0001-05	2x10^6	Puromycin	GFP-T2A-Puro-P2A-Luc	CMV	LFV-V1b	$595		Add to Cart
BHV19400181-6	LFV-0001-06	2x10^6	Puromycin	RFP-T2A-Puro-P2A-Luc	CMV	LFV-V1b	$595		Add to Cart
BHV19400181-7	LFV-0001-07	2x10^6	Blasticidin	GFP-T2A-BSD-P2A-Luc	CMV	LFV-V1b	$595		Add to Cart
BHV19400181-8	LFV-0001-08	2x10^6	Blasticidin	RFP-T2A-BSD-P2A-Luc	CMV	LFV-V1b	$595		Add to Cart
BHV19400181-9	LFV-0001-09	2x10^6	Puromycin	Luc-T2A-GFP-P2A-Puro	EF1a	LFV-V1a	$595		Add to Cart
BHV19400181-10	LFV-0001-10	2x10^6	Blasticidin	Luc-T2A-RFP-P2A-BSD	EF1a	LFV-V1a	$595		Add to Cart
BHV19400181-11	LFV-0001-11	2x10^6	N/A	GFP-P2A-Luc	PGK	LFV-V3	$595		Add to Cart
BHV19400181-12	LFV-0001-12	2x10^6	N/A	RFP-P2A-Luc	PGK	LFV-V3	$595		Add to Cart
BHV19400181-13	LFV-0001-13	2x10^6	N/A	Luc-P2A-GFP	EF1a	LFV-V4	$595		Add to Cart
BHV19400181-14	LFV-0001-14	2x10^6	N/A	Luc-P2A-RFP	EF1a	LFV-V4	$595		Add to Cart

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