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Home / Primary Antibodies / Flow Cytometry (FACS)

UBB Antibody

SKU : BHA10557333

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA316022KA01HU
Alternative Names	UBB; FLJ25987;MGC8385;Ubiquitin B
Categories	Primary Antibodies
Description	Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the ubiquitin-activating enzyme (UBE1 or E1). The activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the ε-amino group of the target protein lysine residue. Combinatorial interactions of different E2 and E3 proteins result in substrate specificity. UBE1 has two isofoms: UBE1a is a nuclear protein of 117 kDa while UBE1b is a nuclear and cytoplasmic protein of 110 kDa.
Host	Rabbit
Intended Use	For research use only. Not for human, diagnostic or therapeutic use.
Isotype	IgG
Raised In	Rabbit
Reactivity	Human, Mouse, Rat
Regulatory	RUO
Relevance	Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the ubiquitin-activating enzyme (UBE1 or E1). The activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the ε-amino group of the target protein lysine residue. Combinatorial interactions of different E2 and E3 proteins result in substrate specificity. UBE1 has two isofoms: UBE1a is a nuclear protein of 117 kDa while UBE1b is a nuclear and cytoplasmic protein of 110 kDa.
Size	100μl(100μg)
Species	Homo Sapiens (Human)
Subcellular Location	Ubiquitin: Cytoplasm, Nucleus

Uniprot	P0CG47
Database Links	HGNC:12463 OMIM:191339 KEGG:hsa:7314 STRING:9606.ENSP00000304697 UniGene:Hs.356190
Function	Ubiquitin
Pathway	Endocytosis
Protein Families	Ubiquitin family

Buffer	Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Format	liquid
Purification	Affinity purification
Purity	Affinity purification
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Storage Buffer	Store at -20oC or -80oC. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Applications

ELISA, WB, IHC, ICC, FC

Description

ELISA ; WB ; IHC ; ICC ; FC, ICC ; IHC ; WB ; FACS ; ELISA

Dilution

WB	1:1000-1:2000
IHC	1:100-1:200
ICC	1:100-1:200
FC	1:50-1:200

Tested Application

ELISA,WB,IHC,ICC,FC;WB:1:1000-1:2000,IHC:1:100-1:200,ICC:1:100-1:200,FC:1:50-1:200

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10557333	CSB-PA316022KA01HU	100μl	$387.33		Add to Cart

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