Fluorescein-Labeled Mouse Anti-Human CD45RA Monoclonal Antibody

Mfr.No.

A6732, A6733, A6740, A6737

Description

CD45RA epitopes are present on all the CD45 restricted molecules in which the exon A is expressed (ABC and AB containing isoforms). Most B lymphocytes express CD45RA isoforms. The differential expression of CD45 isoforms is associated with different functional properties of T lymphocytes. Peripheral T lymphocytes can be divided into two largely distinct populations of CD45RA- CD45RO+ and CD45RA+ CD45RO- cells. CD45RA is expressed by medullar thymocytes and naïve/resting peripheral CD4+ T lymphocytes.

Isotype

mouse IgG2b

Kit Components

The main component of the product is fluorescein-labeled mouse anti-human CD45RA antibody, pH7.2 PBS Buffer, 1% ProClin300.

Specificity	Clone	Subtype	Fluorescence	Λ Ex	Λ Em
CD45RA	HI100	IgG2b	FITC	488nm	525nm
			PE	488nm	575nm
			PE-Cy7	488nm	779nm
			APC-Cy7	633nm	779nm

Test Method

1. Take 2 tubes, add 100 μL of anticoagulated peripheral blood specimen, then add the recommended amount of the same fluorescein-labeled isotype control and this reagent respectively, and incubate at room temperature (18-25℃) for 15-30 minutes, protected from light.
2. Add 2mL of lysis separately, shake well and incubate for 10 minutes at room temperature (18-25℃) away from light.
3. Centrifuge at 300g for 5 minutes, discard the supeatant, add 2mL of PBS buffer to resuspend the cell precipitate, and shake well.
4. Centrifuge at 300g for 5 minutes, discard the supeatant, add 300μL PBS buffer to resuspend the cell precipitate, and incubate for 2 hours at 4°C, protected from light. If delayed detection (more than 2 hours) is required, use 300 μL of PBS buffer containing 1% paraformaldehyde to resuspend the cell precipitate and store at 2-8°C, protected from light, but the fixation time should not exceed 24 hours.

Usage

1. If the flow cytometer is not calibrated, or if the fluorescence compensation is insufficient and the gate is incorrectly positioned, false results may be generated.
2. To obtain the optimum signal-to-noise ratio, the antibodies have been calibrated, so please use the reagents strictly in accordance with their recommended dosage.
3. The data measured with this product are not directly comparable with similar data obtained by other methodological reagents or instruments.

Note

1. Materials such as biological samples, quality control/calibrators, and experimental waste should be handled as potentially infectious materials and disposed of using precautions that comply with regulations.
2. If the concentration of target cells in the sample is too low or too high, special treatment (such as diluting the blood sample or increasing the cell concentration) should be used before staining.
3. This product contains fluorescence. Do not come into direct contact with skin or food and be sure to wear gloves when handling.
4. This product is for research purposes only, not for in vitro diagnostic use.

Clone No	HI100

Storage	2~8℃, protected from light, DO NOT FREEZE.

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Combination SKU	Supplier No.	Size	Specification	Your price
BHA18400166-1	A6732	100T	FITC anti-human CD45RA	$100	Add to Cart
BHA18400166-2	A6733	100T	PE anti-human CD45RA	$100	Add to Cart
BHA18400166-3	A6740	100T	PE-Cy7 anti-human CD45RA	$270	Add to Cart
BHA18400166-4	A6737	100T	APC-Cy7 anti-human CD45RA	$260	Add to Cart

Fluorescein-Labeled Mouse Anti-Human CD45RA Monoclonal Antibody

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