Fluorescein-Labeled Mouse Anti-Human CD4 Monoclonal Antibody

Mfr.No.

A7602, A7603, A3156, A0004, A3155, A3157, A3631

Description

The protein of CD4 is a membrane glycoprotein of T lymphocytes. The CD4 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class II MHC molecules. The CD4 antigen is also a primary receptor for entry of the human immunodeficiency virus through interactions with the HIV Env gp120 subunit. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, granulocytes, as well as in various regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system.

Isotype

mouse IgG1

Kit Components

The main component of the product is fluorescein-labeled mouse anti-human CD4 antibody, pH7.2 PBS Buffer, 1% ProClin300.

Specificity	Clone	Subtype	Fluorescence	Λ Ex	Λ Em
CD3	RPA-T4	IgG1	FITC	488nm	525nm
			PE	488nm	575nm
			PE-QB594	488nm	610nm
			PE-Cy5	488nm	665nm
			PerCP-Cy5.5	488nm	695nm
			APC	633nm	660nm
			APC-Cy7	633nm	779nm
			QB450	405nm	450nm

Test Method

1. Take 2 tubes, add 100 μL of anticoagulated peripheral blood specimen, then add the recommended amount of the same fluorescein-labeled isotype control and this reagent respectively, and incubate at room temperature (18-25℃) for 15-30 minutes, protected from light.
2. Add 2mL of lysis separately, shake well and incubate for 10 minutes at room temperature (18-25℃) away from light.
3. Centrifuge at 300g for 5 minutes, discard the supernatant, add 2mL of PBS buffer to resuspend the cell precipitate, and shake well.
4. Centrifuge at 300g for 5 minutes, discard the supernatant, add 300μL PBS buffer to resuspend the cell precipitate, and incubate for 2 hours at 4°C, protected from light. If delayed detection (more than 2 hours) is required, use 300 μL of PBS buffer containing 1% paraformaldehyde to resuspend the cell precipitate and store at 2-8°C, protected from light, but the fixation time should not exceed 24 hours.

Note

1. If the flow cytometer is not calibrated, or if the fluorescence compensation is insufficient and the gate is incorrectly positioned, false results may be generated.
2. To obtain the optimum signal-to-noise ratio, the antibodies have been calibrated, so please use the reagents strictly in accordance with their recommended dosage.
3. The data measured with this product are not directly comparable with similar data obtained by other methodological reagents or instruments.

Clone No	RPA-T4

Storage	2~8℃, protected from light, DO NOT FREEZE.

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Combination SKU	Supplier No.	Size	Specification	Your price
BHA18400027-1	A7602	100T	FITC anti-human CD4	$100	Add to Cart
BHA18400027-2	A7603	100T	PE anti-human CD4	$150	Add to Cart
BHA18400027-3	A3156	100T	PE-Cy5 anti-human CD4	$220	Add to Cart
BHA18400027-4	A0004	100T	Percp-Cy5.5 anti-human CD4	$170	Add to Cart
BHA18400027-5	A3155	100T	APC anti-human CD4	$90	Add to Cart
BHA18400027-6	A3157	100T	APC-Cy7 anti-human CD4	$250	Add to Cart
BHA18400027-7	A3631	100T	QB450 anti-human CD4	$230	Add to Cart

Fluorescein-Labeled Mouse Anti-Human CD4 Monoclonal Antibody

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