Fluorescein-Labeled Mouse Anti-Human CD38 Monoclonal Antibody

Mfr.No.

A6592, A6600, A6594, 6610697, A6597

Description

The protein of CD38 is a non-lineage-restricted, type II transmembrane glycoprotein that synthesizes and hydrolyzes cyclic adenosine 5'-diphosphate-ribose, an intracellular calcium ion mobilizing messenger. The release of soluble protein and the ability of membrane-bound protein to become intealized indicate both extracellular and intracellular functions for the protein. This protein has an N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites. Crystal structure analysis demonstrates that the functional molecule is a dimer, with the central portion containing the catalytic site. It is used as a prognostic marker for patients with chronic lymphocytic leukemia.

Isotype

mouse IgG1

Kit Components

The main component of the product is fluorescein-labeled mouse anti-human CD38 antibody, pH7.2 PBS Buffer, 1% ProClin300.

Specificity	Clone	Subtype	Fluorescence	Λ Ex	Λ Em
CD38	HIT2	IgG1	FITC	488nm	525nm
			PE-Cy7	488nm	779nm
			PerCP	488nm	670nm
			APC	633nm	660nm
			APC-Cy7	633nm	779nm

Test Method

1. Take 2 tubes, add 100 μL of anticoagulated peripheral blood specimen, then add the recommended amount of the same fluorescein-labeled isotype control and this reagent respectively, and incubate at room temperature (18-25℃) for 15-30 minutes, protected from light.
2. Add 2mL of lysis separately, shake well and incubate for 10 minutes at room temperature (18-25℃) away from light.
3. Centrifuge at 300g for 5 minutes, discard the supeatant, add 2mL of PBS buffer to resuspend the cell precipitate, and shake well.
4. Centrifuge at 300g for 5 minutes, discard the supeatant, add 300μL PBS buffer to resuspend the cell precipitate, and incubate for 2 hours at 4°C, protected from light. If delayed detection (more than 2 hours) is required, use 300 μL of PBS buffer containing 1% paraformaldehyde to resuspend the cell precipitate and store at 2-8°C, protected from light, but the fixation time should not exceed 24 hours.

Usage

1. If the flow cytometer is not calibrated, or if the fluorescence compensation is insufficient and the gate is incorrectly positioned, false results may be generated.
2. To obtain the optimum signal-to-noise ratio, the antibodies have been calibrated, so please use the reagents strictly in accordance with their recommended dosage.
3. The data measured with this product are not directly comparable with similar data obtained by other methodological reagents or instruments.

Note

1. Materials such as biological samples, quality control/calibrators, and experimental waste should be handled as potentially infectious materials and disposed of using precautions that comply with regulations.
2. If the concentration of target cells in the sample is too low or too high, special treatment (such as diluting the blood sample or increasing the cell concentration) should be used before staining.
3. This product contains fluorescence. Do not come into direct contact with skin or food and be sure to wear gloves when handling.
4. This product is for research purposes only, not for in vitro diagnostic use.

Clone No	HIT2

Storage	2~8℃, protected from light, DO NOT FREEZE.

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Combination SKU	Supplier No.	Size	Specification	Your price
BHA18400131-1	A6592	100T	FITC anti-human CD38	$100	Add to Cart
BHA18400131-2	A6600	100T	PE-Cy7 anti-human CD38	$240	Add to Cart
BHA18400131-3	A6594	100T	Percp anti-human CD38	$260	Add to Cart
BHA18400131-4	6610697	100T	APC anti-human CD38	$100	Add to Cart
BHA18400131-5	A6597	100T	APC-Cy7 anti-human CD38	$280	Add to Cart

Fluorescein-Labeled Mouse Anti-Human CD38 Monoclonal Antibody

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