This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
Host
Rabbit
Immunogen
Recombinant full-length human MLANA protein
Isotype
IgG
Positive Control
SK-MEL-13 and SK-MEL-19 Melanoma cell lines; Melanomas
Reactivity
Human, Mouse, Rat
Recombinant
TRUE
Regulatory
RUO
Species
Homo Sapiens (Human)
Swissprot
Q16655
Gene Id
2315
Clone No
MLANA, 1761R
Symbol
MLANA
Weight
20-22kDa (doublet)
Buffer
200μg/ml of Ab purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Applications
FACS, IF, WB, IHC-F
Description
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F
Dilution
Flow Cytometry
0.5-1μg/million cells
Immunofluorescence
0.5-1μg/ml
Western Blotting
0.5-1.0μg/ml
Immunohistology (Formalin-fixed)
0.5-1μg/ml for 30 minutes at RT
No Description Available.
No Description Available.
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No Description Available.
Reviews of Anti-undefined Antibody MLANA,1761R Unconjugated BHA10208246