DOM ; PCWH ; SOX10 ; SRY (sex determining region Y) box 10 ; SRY box containing gene 10 ; SRY related HMG box gene 10 ; Waardenburg syndrome type 2E (WS2E) ; WS4 ; Waardenburg syndrome type 4C (WS4C)
Categories
Primary Antibodies
Cellular Localization
Nuclear
Chromosome Location
22q13.1
Clonality
Monoclonal
Description
SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas.In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.
Host
Rabbit
Immunogen
Recombinant fragment of human SOX10 protein (around aa 115-269) (exact sequence is proprietary)
Isotype
IgG
Positive Control
HepG2 cells. Melanomas, breast carcinomas, gliomas.
Reactivity
Human, Mouse
Recombinant
TRUE
Regulatory
RUO
Species
Homo Sapiens (Human)
Swissprot
P56693
Gene Id
6663
Clone No
SOX10, 2311R
Symbol
SOX10
Weight
49-58kDa
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Applications
FACS, IF, WB, IHC-F
Description
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F
Dilution
Flow Cytometry
0.5-1μg/million cells
Immunofluorescence
1-2μg/ml
Western Blotting
0.5-1.0μg/ml
Immunohistology (Formalin-fixed)
0.5-1μg/ml for 30 minutes at RT
No Description Available.
No Description Available.
Reviews of Anti-undefined Antibody SOX10,2311R Unconjugated BHA10208289