Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI<5.7) and basic (pI>6.0) subfamilies. This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 53kDa (CK13) and 45kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Host
Rabbit
Immunogen
Keratin-enriched preparation from cultured human epithelial cells
Isotype
IgG
Positive Control
A431 cells, Skin, Colon carcinoma
Reactivity
Human, Monkey, Cow, Pig, Goat, Rat, Mouse, Guinea pig, Frog
Recombinant
TRUE
Regulatory
RUO
Species
Homo Sapiens (Human)
Swissprot
P02538
Gene Id
3851
Clone No
KRT, 1877R
Weight
Multiple
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Applications
FACS, IF, WB, IHC-F
Description
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F
Dilution
Flow Cytometry
0.5-1μg/million cells
Immunofluorescence
0.5-1.0μg/ml
Western Blotting
0.5-1.0μg/ml
Immunohistology (Formalin-fixed)
0.5-1.0μg/ml for 30 min at RT
No Description Available.
No Description Available.
Reviews of Anti-undefined Antibody KRT,1877R Unconjugated BHA10208194