This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.
Host
Mouse
Immunogen
Recombinant full-length of human KRT76 protein
Isotype
IgG1 Kappa
Positive Control
PC12 cells, Skin, Prostate carcinoma
Reactivity
Human, Mouse, Rat
Recombinant
TRUE
Regulatory
RUO
Species
Homo Sapiens (Human)
Swissprot
Q01546
Gene Id
51350
Clone No
rKRTH, 2148
Symbol
KRT76
Weight
50-67kDa
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Applications
FACS, IF, WB, IHC-F
Description
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F
Dilution
Flow Cytometry
0.5-1μg/million cells
Immunofluorescence
0.5-1.0μg/ml
Western Blotting
0.5-1.0μg/ml
Immunohistology (Formalin-fixed)
0.25-0.5μg/ml for 30 min at RT
No Description Available.
No Description Available.
Reviews of Anti-undefined Antibody rKRTH,2148 Unconjugated BHA10208190