Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)
Categories
Primary Antibodies
Cellular Localization
Cytoplasmic
Chromosome Location
17q21.31
Clonality
Monoclonal
Description
This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Host
Mouse
Immunogen
Recombinant full-length human GFAP protein
Isotype
IgG1
Positive Control
Brain or Astrocytoma
Reactivity
Human, Mouse, Rat, Cow, Pig, Rabbit, Chicken
Recombinant
TRUE
Regulatory
RUO
Species
Homo Sapiens (Human)
Swissprot
P14136
Gene Id
2670
Clone No
rASTRO, 789
Symbol
GFAP
Weight
~50kDa
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Applications
FACS, IF, WB, IHC-F
Description
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F
Dilution
Flow Cytometry
0.5-1μg/million cells
Immunofluorescence
1-2μg/ml
Western Blotting
0.5-1μg/ml
Immunohistology (Formalin-fixed)
0.25-0.5μg/ml for 30 minutes at RT
No Description Available.
Reviews of Anti-undefined Antibody rASTRO,789 Unconjugated BHA10208212