NEF ; Protein S100-B ; S-100 protein beta chain ; S100 calcium binding protein beta (neural) ; S100 calcium-binding protein B ; S100 protein beta chain ; S100B ; S100beta
Categories
Primary Antibodies
Cellular Localization
Cytoplasmic
Chromosome Location
21q22.3
Clonality
Monoclonal
Description
The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the beta-chain (i.e. in S-100A and S-100B) but not on the alpha-chain of S-100 (i.e. in S-100A and S100A0). This antibody can be used to localize S-100A and S-100B in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.
Host
Mouse
Immunogen
Recombinant full-length human S100B protein
Isotype
IgG2a Kappa
Positive Control
Brain, Melanoma
Reactivity
Human, Mouse, Rat, Cow
Recombinant
TRUE
Regulatory
RUO
Species
Homo Sapiens (Human)
Swissprot
P04271
Gene Id
6285
Clone No
rS100B, 1012
Symbol
S100B
Weight
10-12kDa
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Applications
FACS, IF, WB, IHC-F
Description
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F
Dilution
Flow Cytometry
0.5-1μg/million cells
Immunofluorescence
1-2μg/ml
Western Blotting
0.5-1μg/ml
Immunohistology (Formalin-fixed)
0.25-0.5μg/ml for 30 minutes at RT
No Description Available.
No Description Available.
Reviews of Anti-undefined Antibody rS100B,1012 Unconjugated BHA10208285