Anti-undefined Antibody KRT8.18,2297R Unconjugated BHA10208188

Mfr.No.	RBM37-2297-P1ABX
Alternative Names	CARD2 ; CK8 ; CYK8 ; CYKER ; Cytokeratin Endo A ; DreK8 ; EndoA ; K2C8 ; K8 ; Keratin 8 ; Krt 2.8 ; KRT8 ; Type-II Keratin Kb8. Cell Proliferation-inducing Gene 46 Protein ; CK18 ; CYK18 Cytokeratin Endo B ; K18 ; Keratin-18 ; Kerd ; KRT18
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic
Chromosome Location	17q21.2 (CK8); 12q13.13 (CK18)
Clonality	Monoclonal
Description	Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained. Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.
Host	Rabbit
Immunogen	Recombinant full-length human KRT8 and KRT18 protein
Isotype	IgG
Positive Control	MCF-7 or A431 cells. Skin, Colon, lung or breast carcinoma.
Reactivity	Human
Recombinant	TRUE
Regulatory	RUO
Species	Homo Sapiens (Human)

Swissprot	P05787
Gene Id	3856
Clone No	KRT8.18, 2297R
Symbol	KRT8 ; KRT18
Weight	52.5kDa (CK8); 45kDa (CK18)

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage	Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Applications

FACS, IF, WB, IHC-F

Description

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F

Dilution

Flow Cytometry	0.5-1μg/million cells
Immunofluorescence	0.5-1.0μg/ml
Western Blotting	0.5-1.0μg/ml
Immunohistology (Formalin-fixed)	0.5-1.0μg/ml for 30 min at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10208188	RBM37-2297-P1ABX	100µg	$393.20		Add to Cart

Anti-undefined Antibody KRT8.18,2297R Unconjugated BHA10208188

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