12E7 ; E2 antigen ; MIC 2X ; MIC 2Y ; MIC2 ; Protein MIC2 ; Surface antigen MIC2 ; T-cell surface glycoprotein E2
Categories
Primary Antibodies
Cellular Localization
Cell Surface
Chromosome Location
Xp22.33
Clonality
Monoclonal
Description
Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32). Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis. CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing s sarcoma cells and primitive peripheral neuroectodermal tumors.
Host
Rabbit
Immunogen
Recombinant full-length human MIC2 protein
Isotype
IgG
Positive Control
MOLT-4 cells. Pancreas or Ewing s sarcoma
Reactivity
Human
Recombinant
TRUE
Regulatory
RUO
Species
Homo Sapiens (Human)
Swissprot
P14209
Gene Id
4267
Clone No
MIC2, 1495R
Symbol
CD99
Weight
27-32kDa
Buffer
200μg/ml of Ab purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Applications
FACS, IF, IHC-F
Description
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F
Dilution
Flow Cytometry
2-4μg/million cells
Immunofluorescence
2-4μg/ml
Immunohistology (Formalin-fixed)
5-10μg/ml for 30 min at Room Temp
No Description Available.
No Description Available.
Reviews of Anti-undefined Antibody MIC2,1495R Unconjugated BHA10208156