Anti-undefined Antibody C86,2160R Unconjugated BHA10208149

Mfr.No.	942-RBM4-P1ABX
Alternative Names	Activation B7-2 antigen ; B lymphocyte activation antigen B72 ; CD28 antigen ligand 2 ; CD28LG2 ; CLS1 ; CTLA-4 counter-receptor B7.2 ; Early T-cell co-stimulatory molecule 1 ; ETC-1 ; FUN-1 ; LAB72 ; Ly-58 ; MB7-2 ; TS/A-2
Categories	Primary Antibodies
Cellular Localization	Cell Surface
Chromosome Location	3q13.33
Clonality	Monoclonal
Description	Recognizes a protein of 70kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.
Host	Rabbit
Immunogen	Recombinant full-length human CD86 protein
Isotype	IgG
Positive Control	293T or Jukat cells. Monocytes and dendritic cells, activated T, B and natural killer cells in lymph node or tonsil.
Reactivity	Human, Mouse, Rat
Recombinant	TRUE
Regulatory	RUO
Species	Homo Sapiens (Human)

Swissprot	P4208
Gene Id	942
Clone No	C86, 2160R
Symbol	CD86
Weight	70kDa

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage	Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Applications

FACS, IF, WB, IHC-F

Description

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F

Dilution

Flow Cytometry	0.5-1μg/million cells
Immunofluorescence	0.5-1μg/ml
Western Blotting	0.5-1μg/ml
Immunohistology (Formalin-fixed)	2-4μg/ml for 30 minutes at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10208149	942-RBM4-P1ABX	100µg	$393.20		Add to Cart

Anti-undefined Antibody C86,2160R Unconjugated BHA10208149

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