APY ; ATOPY ; B-lymphocyte antigen CD20 ; B-lymphocyte cell-surface antigen B1 ; Bp35 ; Fc epsilon receptor I beta chain ; Fc Fragment of IgE high affinity I receptor for beta polypeptide ; FCER1B ; High affinity immunoglobulin epsilon receptor subunit beta ; IgE Fc receptor subunit beta ; IGEL ; IGER ; IGHER ; LEU16 ; Leukocyte surface antigen Leu-16 ; Ly44 ; Membrane spanning 4 domains subfamily A member 2 ; Membrane-spanning 4-domains subfamily A member 1 (MS4A1)
Categories
Primary Antibodies
Cellular Localization
Cytoplasmic, Cell Surface
Chromosome Location
11q12.2
Clonality
Monoclonal
Description
Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.
Host
Rabbit
Immunogen
Recombinant full-length human MS4A1 protein
Isotype
IgG
Positive Control
Daudi, Raji, and U266, and human lymphocytes. Lymph nodes and tonsils.
Reactivity
Human
Recombinant
TRUE
Regulatory
RUO
Species
Homo Sapiens (Human)
Swissprot
P11836
Gene Id
931
Clone No
IGEL, 1497R
Symbol
MS4A1
Weight
33-37kDa
Buffer
200μg/ml of Ab purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Applications
FACS, IF, IHC-F
Description
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F
Dilution
Flow Cytometry
0.5-1μg/million cells
Immunofluorescence
0.5-1μg/ml
Immunohistology (Formalin-fixed)
0.5-1μg/ml for 30 minutes at RT
No Description Available.
No Description Available.
Reviews of Anti-undefined Antibody IGEL,1497R Unconjugated BHA10208106