Recognizes a protein of a disulfide-linked 120kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NFkB and SAPK/JNK and induces apoptosis.
Host
Mouse
Immunogen
Recombinant human CD22 protein fragment (around aa 52-178) (exact sequence is proprietary)
Isotype
IgG1
Positive Control
Human peripheral blood lymphocytes. Human tonsil and lymph node.
Reactivity
Human
Recombinant
TRUE
Regulatory
RUO
Species
Homo Sapiens (Human)
Swissprot
P26842
Gene Id
939
Clone No
rLPFS2, 1611
Symbol
CD27
Weight
120kDa
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Applications
FACS, IF, IHC-F
Description
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F
Dilution
Flow Cytometry
0.5-1μg/million cells
Immunofluorescence
0.5-1μg/ml
Immunohistology (Formalin-fixed)
0.5-1μg/ml for 30 minutes at RT
No Description Available.
No Description Available.
Reviews of Anti-undefined Antibody rLPFS2,1611 Unconjugated BHA10208112