Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
Host
Mouse
Immunogen
Recombinant full-length human CNN1 protein
Isotype
IgG1 Kappa
Positive Control
Myoepithelial cells in breast ducts
Reactivity
Human, Rat
Recombinant
TRUE
Regulatory
RUO
Species
Homo Sapiens (Human)
Swissprot
P51911
Gene Id
1264
Clone No
rCNN1, 832
Symbol
CNN1
Weight
34kDa
Buffer
200μg/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Applications
FACS, IF, IHC-F
Description
Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F
Dilution
Flow Cytometry
0.5-1μg/million cells
Immunofluorescence
1-2μg/ml
Immunohistology (Formalin-fixed)
0.5-1μg/ml for 30 minutes at RT
No Description Available.
No Description Available.
Reviews of Anti-undefined Antibody rCNN1,832 Unconjugated BHA10208090