It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Host
Rabbit
Immunogen
Biotinylated sheep immunoglobulin
Isotype
IgG
Positive Control
Biotinylated proteins in solution or on tissues
Reactivity
All species
Recombinant
TRUE
Regulatory
RUO
Clone No
BTN, 2032R
Weight
244Da
Buffer
200μg/ml of Ab purified by Protein A Column. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Applications
CM, FACS, IF, WB, IHC-F
Description
No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.Optimal dilution for a specific application should be determined., CM ; FACS ; IF ; WB ; IHC-F
Dilution
Confocal Microscopy
Flow Cytometry
0.5-1μg/million cells
Immunofluorescence
0.5-1μg/ml
Western Blotting
Immunohistology (Formalin-fixed)
0.5-1μg/ml for 30 minutes at RT
No Description Available.
No Description Available.
Reviews of Anti-undefined Antibody BTN,2032R Unconjugated BHA10208084