Anti-undefined Antibody MLANA,1409R Unconjugated BHA10207989

Mfr.No.	2315-RBM9-P0
Alternative Names	Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA
Antigen	MART-1 / Melan-A / MLANA
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic
Chromosome Location	9p24.1
Clonality	Monoclonal
Conjugate	Unconjugated
Description	This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
Host	Rabbit
Immunogen	Recombinant full-length human MLANA protein
Isotype	IgG
Positive Control	SK-MEL-13 and SK-MEL-19 Melanoma cell lines; Melanomas
Reactivity	Human, Mouse, Rat, Dog
Recombinant	TRUE
Regulatory	RUO
Species	Homo Sapiens (Human)

Swissprot	Q16655
Gene Id	2315
Clone No	MLANA, 1409R
Symbol	MLANA
Weight	20-22kDa (doublet)

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	200μg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab with BSA and Azide at 200μg/ml
Storage	Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Applications

FACS, IF, WB, IHC-F

Description

Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F

Dilution

Flow Cytometry	0.5-1μg/million cells
Immunofluorescence	0.5-1μg/ml
Western Blotting	0.5-1.0μg/ml
Immunohistology (Formalin-fixed)	0.5-1μg/ml for 30 minutes at RT