Anti-undefined Antibody S100B,1706R Unconjugated BHA10208028

Mfr.No.	6285-RBM4-P0
Alternative Names	NEF ; Protein S100-B ; S-100 protein beta chain ; S100 calcium binding protein beta (neural) ; S100 calcium-binding protein B ; S100 protein beta chain ; S100B ; S100beta
Antigen	S100B
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic
Chromosome Location	21q22.3
Clonality	Monoclonal
Conjugate	Unconjugated
Description	S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the beta-chain (i.e. in S-100A and S-100B) but not on the alpha-chain of S-100 (i.e. in S-100A and S100A0). This antibody can be used to localize S-100A and S-100B in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.
Host	Rabbit
Immunogen	Recombinant full-length human S100B protein
Isotype	IgG
Positive Control	Brain, Melanoma
Reactivity	Human, Mouse, Rat, Cow
Recombinant	TRUE
Regulatory	RUO
Species	Homo Sapiens (Human)

Swissprot	P04271
Gene Id	6285
Clone No	S100B, 1706R
Symbol	S100B
Weight	10-12kDa

Buffer	200μg/ml of Ab purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	200μg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab with BSA and Azide at 200μg/ml
Storage	Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Applications

FACS, IF, WB, IHC-F

Description

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F

Dilution

Flow Cytometry	0.5-1μg/million cells
Immunofluorescence	1-2μg/ml
Western Blotting	0.5-1μg/ml
Immunohistology (Formalin-fixed)	0.25-0.5μg/ml for 30 minutes at RT