Anti-undefined Antibody GPC3,1534R Unconjugated BHA10207959

Mfr.No.	2719-RBM4-P0
Alternative Names	DGSX ; Glypican proteoglycan 3 ; GPC3 ; GTR2-2 ; Heparan sulphate proteoglycan ; Intestinal protein OCI-5 ; MXR7 ; OCI-5 ; SDYS ; Secreted glypican-3 ; SGBS1
Antigen	Glypican-3 (GPC3)
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic
Chromosome Location	Xq26.2
Clonality	Monoclonal
Conjugate	Unconjugated
Description	Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilm s tumor. In patients with HCC, GPC3 is overexpressed in neoplastic liver tissue and elevated in serum, but is undetectable in normal liver, benign liver, and the serum of healthy donors. GPC3 expression is also found to be higher in HCC liver tissue than in cirrhotic liver or liver with focal lesions such as dysplastic nodules and areas of hepatic adenoma (HA) with malignant transformation. In the context of testicular germ cell tumors, GPC3 expression is up regulated in certain histologic subtypes, specifically yolk sac tumors and choriocarcinoma. A high level of GPC3 expression is also found in some types of embryonal tumors, such as Wilm s tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100% in follicular carcinoma and 70% in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.
Host	Rabbit
Immunogen	Recombinant full-length human GPC3 protein
Isotype	IgG
Positive Control	293T cells or Hepatocellular carcinoma
Reactivity	Human, Rat
Recombinant	TRUE
Regulatory	RUO
Species	Homo Sapiens (Human)

Swissprot	P51654
Gene Id	2719
Clone No	GPC3, 1534R
Symbol	GPC3
Weight	67kDa

Buffer	200μg/ml of Ab purified by Protein A. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	200μg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab with BSA and Azide at 200μg/ml
Storage	Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Applications

FACS, IF, IHC-F

Description

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with1mM EDTA buffer, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F

Dilution