Anti-undefined Antibody PTPRC,1783R Unconjugated BHA10207881

Mfr.No.	5788-RBM23-P0
Alternative Names	B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic, Cell Surface
Chromosome Location	1q31.3
Clonality	Monoclonal
Description	CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin.
Host	Rabbit
Immunogen	Human B-lymphocytes
Isotype	IgG
Positive Control	Ramos, U-698, or GA-10 cells. Tonsil
Reactivity	Human
Recombinant	TRUE
Regulatory	RUO

Swissprot	P08575
Gene Id	5788
Clone No	PTPRC, 1783R
Symbol	PTPRC
Weight	180-220kDa

Buffer	200μg/ml of Ab purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	200μg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab with BSA and Azide at 200μg/ml
Storage	Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Applications

FACS, IF, WB, IHC-F

Description

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F

Dilution

Flow Cytometry	0.5-1μg/million cells
Immunofluorescence	0.5-1μg/ml
Western Blotting	0.5-1.0μg/ml
Immunohistology (Formalin-fixed)	0.5-1.0μg/ml for 30 minutes at RT