Anti-undefined Antibody IGEL,1497R Unconjugated BHA10207848

Mfr.No.	931-RBM7-P0
Alternative Names	APY ; ATOPY ; B-lymphocyte antigen CD20 ; B-lymphocyte cell-surface antigen B1 ; Bp35 ; Fc epsilon receptor I beta chain ; Fc Fragment of IgE high affinity I receptor for beta polypeptide ; FCER1B ; High affinity immunoglobulin epsilon receptor subunit beta ; IgE Fc receptor subunit beta ; IGEL ; IGER ; IGHER ; LEU16 ; Leukocyte surface antigen Leu-16 ; Ly44 ; Membrane spanning 4 domains subfamily A member 2 ; Membrane-spanning 4-domains subfamily A member 1 (MS4A1)
Antigen	CD20 / MS4A1
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic, Cell Surface
Chromosome Location	11q12.2
Clonality	Monoclonal
Conjugate	Unconjugated
Description	Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.
Host	Rabbit
Immunogen	Recombinant full-length human MS4A1 protein
Isotype	IgG
Positive Control	Daudi, Raji, and U266, and human lymphocytes. Lymph nodes and tonsils.
Reactivity	Human
Recombinant	TRUE
Regulatory	RUO
Species	Homo Sapiens (Human)

Swissprot	P11836
Gene Id	931
Clone No	IGEL, 1497R
Symbol	MS4A1
Weight	33-37kDa

Buffer	200μg/ml of Ab purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	200μg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab with BSA and Azide at 200μg/ml
Storage	Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Applications

FACS, IF, IHC-F

Description

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F

Dilution