Anti-Cytokeratin, pan Antibody BHA10207786

Mfr.No.	RBP35-1835-P1ABX
Alternative Names	K1B ; KRT1B ; Keratin, type II cytoskeletal 1b ; K77 ; CK-1B ; Keratin 1B ; Keratin-77 ; Cytokeratin-1B ; Type-II Keratin Kb39
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic
Chromosome Location	17q21.2 (KRT77) ; 12q13.13 (KRT76)
Clonality	Polyclonal
Description	Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. It is a broad spectrum anti pan-cytokeratin antibody, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It may be useful to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has high sensitivity in the recognition of epithelial cells and carcinomas.
Host	Rabbit
Immunogen	Recombinant full-length human KRT76 and KRT77 proteins
Positive Control	Skin, Adeno- or Squamous carcinomas
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	Q7Z794 (KRT77) and Q01546 (KRT76)
Uniprot	334989 (KRT77) and 654392 (KRT76)
Gene Id	374454 (KRT77) and 51350 (KRT76)

Buffer	200μg/ml of Ab purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, WB, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F

Dilution

FACS	0.5-1μg/million cells
IF	1-2μg/ml
WB	0.5-1μg/ml for 2 hours at RT
IHC-F	0.25-0.5μg/ml for 30 min at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10207786	RBP35-1835-P1ABX	100µg	$393.20		Add to Cart

Anti-Cytokeratin, pan Antibody BHA10207786

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