It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Host
Mouse
Immunogen
Bromodeoxyuridine (BrdU) conjugated to hemocyanine (isolated from Helix pomatia)
Positive Control
Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU
Reactivity
All species
Recombinant
FALSE
Regulatory
RUO
Swissprot
Not Applicable
Uniprot
Not Applicable
Gene Id
Not Applicable
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, IHC-F
Description
Staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F
Dilution
FACS
0.5-1μg/million cells in 0.1ml
IF
0.5-1μg/ml
IHC-F
0.5-1μg/ml for 30 minutes at RT
Reviews of Anti-Bromodeoxyuridine (BrdU) Antibody BHA10207727